prikaz prve stranice dokumenta Effects of newly designed albumin-derived perfluorocarbon-based nanoparticles in the therapy of decompression induced gas embolisms in rats
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doctoral thesis
Effects of newly designed albumin-derived perfluorocarbon-based nanoparticles in the therapy of decompression induced gas embolisms in rats
2023. urn:nbn:hr:171:644311
Mayer, Dirk
University of Split
School of Medicine

Cite this document

Mayer, D. (2023). Effects of newly designed albumin-derived perfluorocarbon-based nanoparticles in the therapy of decompression induced gas embolisms in rats (Doctoral thesis). Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:644311

Mayer, Dirk. "Effects of newly designed albumin-derived perfluorocarbon-based nanoparticles in the therapy of decompression induced gas embolisms in rats." Doctoral thesis, University of Split, School of Medicine, 2023. https://urn.nsk.hr/urn:nbn:hr:171:644311

Mayer, Dirk. "Effects of newly designed albumin-derived perfluorocarbon-based nanoparticles in the therapy of decompression induced gas embolisms in rats." Doctoral thesis, University of Split, School of Medicine, 2023. https://urn.nsk.hr/urn:nbn:hr:171:644311

Mayer, D. (2023). 'Effects of newly designed albumin-derived perfluorocarbon-based nanoparticles in the therapy of decompression induced gas embolisms in rats', Doctoral thesis, University of Split, School of Medicine, accessed 27 April 2024, https://urn.nsk.hr/urn:nbn:hr:171:644311

Mayer D. Effects of newly designed albumin-derived perfluorocarbon-based nanoparticles in the therapy of decompression induced gas embolisms in rats [Doctoral thesis]. Split: University of Split, School of Medicine; 2023 [cited 2024 April 27] Available at: https://urn.nsk.hr/urn:nbn:hr:171:644311

D. Mayer, "Effects of newly designed albumin-derived perfluorocarbon-based nanoparticles in the therapy of decompression induced gas embolisms in rats", Doctoral thesis, University of Split, School of Medicine, Split, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:171:644311

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Metadata
TitleEffects of newly designed albumin-derived perfluorocarbon-based nanoparticles in the therapy of decompression induced gas embolisms in rats
Title (croatian)Učinci novih perfluorokarbonskih nanočestica stvorenih pomoću albumina u liječenju plinskih embolizama uzrokovanih dekompresijom u štakora
AuthorDirk Mayer https://orcid.org/0000-0003-2959-7923
MentorKatja Bettina Ferenz https://orcid.org/0000-0001-6011-2467 (mentor)
Committee memberZoran Valić (predsjednik povjerenstva)
Committee memberJoško Božić (član povjerenstva)
Committee memberAnita Markotić (član povjerenstva)
GranterUniversity of Split
School of Medicine
Split
Defense date and country2023-12-14, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Human Physiology
Universal decimal classification
(UDC)		616 - Pathology. Clinical medicine
Abstract
For three decades, studies have attempted to demonstrate the therapeutic efficacy of perfluorocarbons (PFCs) in reducing the onset of decompression trauma. However, none of these emulsion-based preparations are accepted for therapeutic use in the western world, mainly because of severe side-effects and a long organ retention time. A new development to guarantee a stable dispersion without these disadvantages is the encapsulation of PFCs in nanocapsules with an albumin shell. Purpose: Newly designed albumin-derived perfluorocarbon-based artificial oxygen carriers (AAOCs) are used in a rodent in-vivo model as a preventive therapy for decompression sickness (DCS). Methods: Thirty-seven rats were treated with either A-AOCs (n=12), albumin nanocapsules filled with neutral oil (A-O-N, n=12) or 5% human serum albumin solution (A-0-0, n=13) before a simulated dive. Eleven rats, injected with A-AOCs, stayed at normal pressure (A-AOCssurface). Clinical, laboratory and histological evaluations were performed. Results: The occurrence of DCS depended on the treatment group. A-AOCs significantly reduced DCS-appearance and mortality. Furthermore, a significant improvement of survival rate was found (A-AOCs compared with A-0-0). Histological assessment of A-AOCs-dive compared with A-0-0-dive animals revealed significantly higher accumulation of macrophages, but less blood congestion in the spleen and significantly less hepatic circulatory disturbance, vacuolisation, and cell damage. Compared to non-diving controls lactate and myoglobin showed a significant increase in the A-0-0- but not in the A-AOCs-dive group. Conclusion: Intravenous application of A-AOCs was well tolerated and effective in reducing the occurrence of DCS, animals showed significantly higher survival rates and less symptoms compared to the albumin group (A-0-0). Analysis of histological results and fast reacting plasma parameters confirmed the preventive properties of A-AOCs.
Abstract (croatian)
Istraživanja kojima se ispituje moguća terapijska učinkovitost perfluorokarbona (PFC) u smanjenju dekompresijske traume traju već više od tri desetljeća. Unatoč tome, niti jedan od PFC emulzijskih pripravaka nije odobren u terapijske svrhe u zapadnom svijetu, uglavnom zbog ozbiljnih nuspojava i produljenog zadržavanja u raznim organima. Nova vrsta PFC pripravka, u obliku nanokapsula s albuminskom ovojnicom, bi mogla omogućiti stabilnu smjesu bez ovakvih nedostataka. Svrha: Ispitati novu vrstu prenositelja kisika (A-AOC), koji su bazirani na PFC tehnologiji i uključuju albuminsku ovojnicu, kao moguću preventivnu terapiju u štakorskom in vivo modelu dekompresijske bolesti (DCS). Metode: Trideset sedam štakora je tretirano jednim od sljedećih pripravaka: A-AOC (n=12), albuminskim nanokapsulama ispunjenima neutralnim lipidima (A-O-N, n=12) ili 5 % otopinom albumina ljudskog seruma (A-0-0), a prije simuliranog zarona. Dodatnih jedanaest štakora je primilo A-AOC, ali su potom bili izloženi normalnom tlaku zraka (A-AOC-Površina). Potom je učinjena njihova klinička, laboratorijska i histološka analiza. Rezultati: Pojava dekompresijske bolesti je ovisila o vrsti tretmana. U A-AOC štakora je primijećena značajno smanjena incidencija DCS-a i mortaliteta (u usporedbi s A-0-0 štakorima). Histološka procjena A-AOC štakora, u usporedbi s A-0-0 štakorima, je pokazala značajno veće nakupljanje makrofaga, ali i manju kongestiju krvi u slezeni, kao i značajno manje poremećaje u jetrenoj cirkulaciji, stvaranju vakuola i oštećenju stanica. U usporedbi s kontrolnim životinjama (bez zarona), zabilježen je značajan porast laktata i mioglobina u A-0- 0 grupi životinja, ali ne i u A-AOC grupi. Zaključak: Eksperimentalne životinje su pokazale dobru toleranciju na intravensku primjenu AAOC, koja se pokazala i učinkovitom u smanjenju pojavnosti DCS-a, povećanju preživljenja i težini simptoma, u usporedbi s grupom životinja tretiranih albuminom (A-0-0). Analize histoloških rezultata i brzo reagirajućih parametara iz plazme su potvrdile preventivne osobine A-AOC pripravka.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:171:644311
Promotion2023
Study programmeTitle: Evidence-Based Clinical Medicine
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-12-07 13:39:20