CILJ ISTRAŽIVANJA: Cilj istraživanja je otkriti postoji li značajna razlika u izražaju proupalnih citokina TNF-α, IL-6 i IL-1β u tumorskom tkivu bubrega u odnosu na zdravo bubrežno tkivo. USTROJ ISTRAŽIVANJA: Presječna studija. MJESTO ISTRAŽIVANJA: Istraživanje je provedeno u laboratoriju Katedre za imunologiju i genetiku, Medicinskog fakulteta u Splitu i na Odjelu za urologiju, Kliničkog bolničkog centra Split. SUDIONICI: Istraživanjem je obuhvaćeno 24 uzorka bubrežnog tkiva. Od toga je bilo 12 uzoraka zdravog bubrežnog tkiva i 12 uzoraka adenokarcinoma bubrega gradusa 2. Svi uzorci uzeti su operacijskim zahvatom na Odjelu za urologiju, KBC-a Split. GLAVNA MJERA ISHODA: Glavna mjera ishoda je povećani izražaj proupalnih citokina u tkivu adenokarcinoma bubrega, dokazana reakcijom RT-PCR. REZULTATI: Istraživanjem je obuhvaćeno 24 uzorka tkiva. Od toga je bilo 12 tumorskog tkiva, a 12 zdravog tkiva bubrega. Obradom podataka, dobili smo statistički značajan porast IL-6 u tumorskom tkivu bubrega (P<0,05). Vrijednosti porasta TNF-α i IL-1β nisu bile statistički značajne. ZAKLJUČCI: Značajan porast IL-6 u tumorskom tkivu bubrega upućuje na povezanost upale i nastanka karcinoma bubrega svijetlih stanica.

OBJECTIVE: The aim of the study is to determine expression level of proinflammatory cytokines TNF-α, IL-6 and IL-1β in renal cell carcinoma (compared to the healthy kidney tissue). DESIGN: The study was designed as a cross-sectional study. SETTINGS: The study was carried out at the Department of Imunology and Genetics, at the Medical University Split and the Department of Urology, University Hospital Centre Split. PARTICIPANTS: The study consisted 12 was renal cell carcinoma tissue, and the 12 healthy kidney tissue samples. All of the samples were collected during an operational procedure at the Department of Urology, University Hospital Centre Split. MAIN OUTCOME MEASURES: Main outcome measure is a elevated expression of proinflammatory cytokines in the renal cell carcinoma tissue, which has been detected by RT-PCR approach. RESULTS: We found a statistically significant increase of IL-6 expression in the tumor tissue, compared to the healthy kidney tissue (P<0,05). The increase in expression of TNF-α and IL-1β was not statistically significant. CONCLUSIONS: We found a significant increase in IL-6 in tumor tissue, which indicates connection between inflammation and renal carcinogenesis.