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master's thesis
Izražaj p16/Ki-67 u atipičnim skvamoznim stanicama i skvamoznim intraepitelnim lezijama niskog stupnja
2021. urn:nbn:hr:171:017071
Marušić, Antonija
University of Split
School of Medicine

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Marušić, A. (2021). Izražaj p16/Ki-67 u atipičnim skvamoznim stanicama i skvamoznim intraepitelnim lezijama niskog stupnja (Master's thesis). Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:017071

Marušić, Antonija. "Izražaj p16/Ki-67 u atipičnim skvamoznim stanicama i skvamoznim intraepitelnim lezijama niskog stupnja." Master's thesis, University of Split, School of Medicine, 2021. https://urn.nsk.hr/urn:nbn:hr:171:017071

Marušić, Antonija. "Izražaj p16/Ki-67 u atipičnim skvamoznim stanicama i skvamoznim intraepitelnim lezijama niskog stupnja." Master's thesis, University of Split, School of Medicine, 2021. https://urn.nsk.hr/urn:nbn:hr:171:017071

Marušić, A. (2021). 'Izražaj p16/Ki-67 u atipičnim skvamoznim stanicama i skvamoznim intraepitelnim lezijama niskog stupnja', Master's thesis, University of Split, School of Medicine, accessed 13 April 2024, https://urn.nsk.hr/urn:nbn:hr:171:017071

Marušić A. Izražaj p16/Ki-67 u atipičnim skvamoznim stanicama i skvamoznim intraepitelnim lezijama niskog stupnja [Master's thesis]. Split: University of Split, School of Medicine; 2021 [cited 2024 April 13] Available at: https://urn.nsk.hr/urn:nbn:hr:171:017071

A. Marušić, "Izražaj p16/Ki-67 u atipičnim skvamoznim stanicama i skvamoznim intraepitelnim lezijama niskog stupnja", Master's thesis, University of Split, School of Medicine, Split, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:171:017071

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Metadata
TitleIzražaj p16/Ki-67 u atipičnim skvamoznim stanicama i skvamoznim intraepitelnim lezijama niskog stupnja
Title (english)Expression of p16/ Ki-67 in atypical squamous cells and low-grade squamous intra-epithelial lesions
AuthorAntonija Marušić
MentorDinka Šundov (mentor)
Committee memberKatarina Vilović (predsjednik povjerenstva)
Committee memberSandra Zekić Tomaš (član povjerenstva)
Committee memberIvana Pavlinac Dodig (član povjerenstva)
GranterUniversity of Split
School of Medicine
Split
Defense date and country2021-07-16, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Cytology, Histology and Embryology
Abstract
CILJ ISTRAŽIVANJA: Prikazati izražaj p16/Ki-67 te morfološke osobitosti u atipičnim skvamoznim stanicama i skvamoznim intraepitelnim lezijama niskog stupnja u citološkim obriscima vrata maternice. ISPITANICI I METODE: Retrospektivno istraživanje provedeno je na Kliničkom zavodu za patologiju, sudsku medicinu i citologiju Kliničkog bolničkog centra Split. U studiju su uključene 264 pacijentice kojima je napravljen CINtec® PLUS Cytology test, dvostruko imunocitokemijsko bojanje na p16 i Ki-67 u razdoblju od 1. siječnja 2019 do 31. prosinca 2020. Uvidom u medicinsku dokumentaciju prikupljeni su podatci o nalazima cervikalnog obriska te rezultatima CINtec® PLUS Cytology testa. REZULTATI: Prosječna dob pacijentica bila je 39 godina ± 15.399 (medijan: 37; raspon: 17-82 god.). Najzastupljenija dijagnoza bio je LSIL, odnosno pločasta intraepitelna lezija niskog stupnja koju je imalo 48,5% svih pacijentica. Od 264 ispitanice njih 2,88% bilo je CINtec® pozitivno. CINtec® negativnih bilo je 83,71%. Statističkom analizom podataka dokazali smo da udio pozitivnih rezultata CINtec® PLUS Cytology testa raste s gradusom citološke i patohistološke dijagnoze. Citološki dokaz HPV infekcije bio je pozitivan u 26,14% ispitanica, a najviše pozitivnih bilo je u skupini s LSIL-om (52,34%). U skupini ispitanica s HSIL-om te u skupini SCC najveći udio ispitanica (100%) je imalo pozitivan nalaz koilocitoze. Perzistentne citološke abnormalnosti imalo je 87,0% kontrolnih PAPA testova. Također, uočeno je da pacijentice s koilocitozom imaju veće šanse za citološku dijagnozu višeg gradusa te perzistentne abnormalnosti. ZAKLJUČAK: Ovim istraživanjem ustanovili smo da je CINtec® PLUS Cytology probir korisna dodatna metoda u sekundarnom screeningu prije podvrgavanja daljnjim pretragama u vidu kolposkopije i biopsije posebno u mladih žena s pozitivnim HR-HPV testom, ali da PAPA test ipak ostaje zlatni standard u ranom otkrivanju malignih bolesti vrata maternice.
Abstract (english)
OBJECTIVES: The main purpose of this study was to determine the expression of p16/Ki-67 and morphological characteristics in atypical squamous cells and low-grade squamous intra-epithelial lesions. SUBJECTS AND METHODS: A retrospective study was conducted at the Clinical Department of Pathology, Forensic Medicine and Cytology of the University Hospital of Split. This study included 264 patients whos samples underwent immunostaining by CINtec® PLUS Cytology test in the time period from 1st of January 2019 til 31st of December 2020. By inspecting medical documentation results of conventional cervical smear and results of CINtec® PLUS Cytology test were collected. RESULTS: Among the patients, average age was 39 years ± 15.399 (median: 37; range: 17-82 years). Most common diagnosis was LSIL, low-grade squamous intra-epithelial lesion, present in 48,5% of all patients. Among 264 women 2,88% were CINtec® positive and 83,71%. were CINtec® negative. By statistical analysis we have determined that the share of CINtec® PLUS Cytology positive results increases according to cytological and pathohistological diagnosis. Cytological evidence of HPV infection was present in 26,14% of women. All patients with HSIL or SCC had koilocytosis, and 52,34% of patients with LSIL had koilocytosis. 87,0% of follow up Pap smears had persistent cytological abnormalities. We have also concluded that the patients with koilocytosis have greater odds of having higher grade cytological diagnosis and persistent abnormalities. CONCLUSION: With this research we have established that CINtec® PLUS Cytology dual-stain is a useful tool in secondary screening before undergoing further examinations such as colposcopies and biopsies, especially in young HR-HPV positive women. Regardless, conventional Pap smear remains the golden standard in the early detection of cervical malignancies.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:171:017071
Study programmeTitle: Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-07-20 06:34:07