Objectives: Main goal of this research was to assess knowledge and attitudes of students from the Study of Dental Medicine Medicine, University of Split School of Medicine on the use of the PRGF method in everyday practice. Materials and methods: Fifth and sixth year students participated in this research. The research was conducted using a questionnaire created and shared via a Google form. The questionnaire consisted of two parts. The first part of the questionnaire contained questions about the year of study that the student attends, the frequency of attending the congress on the application of the PRGF method, and questions about the representation of this topic during classes, as well as the frequency of attendance at the PRGF method. In the second part of the questionnaire, students' knowledge of the application of the PRGF method and its effect was assessed using a linear scale with values from 1 to 5 (Likert scale). Descriptive statistics were used in the data analysis. The Mann-Whitney U Test was used to investigate potential differences in responses between the two student years. To investigate the potential influence of predictor variables, multiple regression analysis and a general regression model were used, the results of which are presented in the form of Pareto t-value diagrams. Results: Overall 80 students took part in this survey, of which 41 were fifth-year and 29 were sixth-year students. A significantly higher number of fifth-year students (78%) attended congresses or webinars on the topic of PRGF compared to sixth-year students (7%). Likewise, a higher number of fifth-year students (61%) follow the literature at PRGF compared to sixth-year students (45%). 90% of fifth-year students and 72% of sixth-year students were introduced to PRGF during their studies, most often as part of the Oral Surgery and Periodontology courses. An equal number of students from the fifth (51%) and sixth year (48%) during the study attended a procedure during which the PRGF method was used, and almost all students would like to use the PRGF method in their future work. Students in both years of study believe that PRGF technology has a special benefit in periodontology, oral surgery, dermatology, and maxillofacial surgery. In case of the question "Does the application of the PRGF method lead to the absence of infection?" students of both academic years showed a poor level of knowledge, where 22% of fifth-year students and 21% of sixth- year students answered correctly. Very poor results were also obtained in the case of the question "Does PRGF technology stimulate angiogenesis?" where only 17% of fifth-year students and 10% of sixth-year students answered correctly. Poor results were also on the question "Does PRGF technology stimulate chemotaxis?", where 39% of respondents in the fifth year and only 24% of respondents in the sixth year answered correctly. Both academic years, 83% of fifth-year students and 76% of sixth- year students, believe that they are partially familiar with the procedure of the PRGF method (only with the basic elements). However, only 17% of fifth-year students and only 7% of sixth-year students are fully familiar with the procedure of the PRGF method. To the question "Do you think that the topic of PRGF should be more involved in the teaching of our faculty?" a total of 99% of students from both academic years answered in the affirmative. Also, the same percentage of students want additional education about this method. Conclusions: Most students of Dental Medicine at the Faculty of Medicine in Split have only basic knowledge about the application of the PRGF method, and very few students are fully familiar with the procedure of the PRGF method. Given that fifth- year students participated in congresses and had additional practices on the topic of the PRGF method, their knowledge is better. All students are willing to know more about the application of the PRGF method, which confirms that there is a need to introduce additional education on this topic in the teaching of future dental medicine doctors.