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master's thesis
Izražaj vaskularnog endotelnog čimbenika rasta i receptora čimbenika rasta fibroblasta 1 i 2 u karcinomu bubrega
2020. urn:nbn:hr:171:925999
Ćorluka, Iva
University of Split
School of Medicine
Department of Anatomy, Histology and Embryology

Cite this document

Ćorluka, I. (2020). Izražaj vaskularnog endotelnog čimbenika rasta i receptora čimbenika rasta fibroblasta 1 i 2 u karcinomu bubrega (Master's thesis). Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:925999

Ćorluka, Iva. "Izražaj vaskularnog endotelnog čimbenika rasta i receptora čimbenika rasta fibroblasta 1 i 2 u karcinomu bubrega." Master's thesis, University of Split, School of Medicine, 2020. https://urn.nsk.hr/urn:nbn:hr:171:925999

Ćorluka, Iva. "Izražaj vaskularnog endotelnog čimbenika rasta i receptora čimbenika rasta fibroblasta 1 i 2 u karcinomu bubrega." Master's thesis, University of Split, School of Medicine, 2020. https://urn.nsk.hr/urn:nbn:hr:171:925999

Ćorluka, I. (2020). 'Izražaj vaskularnog endotelnog čimbenika rasta i receptora čimbenika rasta fibroblasta 1 i 2 u karcinomu bubrega', Master's thesis, University of Split, School of Medicine, accessed 04 December 2024, https://urn.nsk.hr/urn:nbn:hr:171:925999

Ćorluka I. Izražaj vaskularnog endotelnog čimbenika rasta i receptora čimbenika rasta fibroblasta 1 i 2 u karcinomu bubrega [Master's thesis]. Split: University of Split, School of Medicine; 2020 [cited 2024 December 04] Available at: https://urn.nsk.hr/urn:nbn:hr:171:925999

I. Ćorluka, "Izražaj vaskularnog endotelnog čimbenika rasta i receptora čimbenika rasta fibroblasta 1 i 2 u karcinomu bubrega", Master's thesis, University of Split, School of Medicine, Split, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:171:925999

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Metadata
TitleIzražaj vaskularnog endotelnog čimbenika rasta i receptora čimbenika rasta fibroblasta 1 i 2 u karcinomu bubrega
Title (english)The expression of vascular endothelial growth factor and fibroblast growth factor receptors 1 and 2 in renal cell carcinoma
AuthorIva Ćorluka
MentorKatarina Vukojević (mentor)
Committee memberNatalija Filipović (predsjednik povjerenstva)
Committee memberSnježana Mardešić (član povjerenstva)
Committee memberDarko Kero (član povjerenstva)
GranterUniversity of Split
School of Medicine
(Department of Anatomy, Histology and Embryology)
Split
Defense date and country2020, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Cytology, Histology and Embryology
Abstract
Uvod: Karcinom bubrežnih stanica (RCC) najčešći je karcinom bubrega s kontinuiranim porastom incidencije i smrtnosti u svijetu. Stoga su napori brojnih istraživačkih skupina usmjereni na otkrivanje značajnih dijagnostičkih, prognostičkih i terapijskih biljega koji bi poboljšali kvalitetu zdravstvene usluge za pacijente oboljele od karcinoma bubrežnih stanica. Ciljevi: Ciljevi našeg istraživanja bili su utvrđivanje izražaja VEGF, FGFR1 i FGFR2 biljega u različitim stadijima karcinoma bubrežnih stanica u odnosu na izražaj u normalnom tkivu te utvrđivanje kolokalizacije VEGF-a s FGFR1 i FGFR2 u karcinomu bubrega s obzirom na patohistološki stupanj tumora. Materijali i metode: Istraživanje smo proveli na uzorcima tumorskog tkiva i tkiva zdravog ruba bubrega, uzetim od ispitanika kojima je dijagnosticiran karcinom bubrega niskog (5 uzoraka) i visokog (5 uzoraka) stupnja. Provedeno je dvostruko imunofluorescencijsko bojenje tkivnih rezova karcinoma bubrega i kontrola na kombinaciju VEGF-a s FGFR1 i FGFR2. Postotak pozitivnih stanica izračunali smo na deset reprezentativnih polja iz svakog reza i izrazili kao srednju vrijednost ± SD, a u odnosu između skupina (niski i visoki stadij te kontrolna skupina). Za analizu podataka koristili smo Kruskal-Wallis i Dunn posthoc test. Rezultati: Rezultati našeg istraživanja pokazali su pozitivan imunohistokemijski izražaj VEGF, FGFR1 i FGFR2 u tkivu karcinoma bubrega niskog i visokog stupnja, kao i u normalnom bubrežnom tkivu. Ustanovljena je povremena kolokalizacija FGFR1 i VEGF u normalnom bubrežnom tkivu te kolokalizacija FGFR1 i FGFR2 s VEGF kod karcinoma bubrega niskog i visokog stupnja. U normalnom bubrežnom tkivu, FGFR1, FGFR2 i VEGF pokazali su blagu reaktivnost. U tkivu karcinoma bubrega niskog stupnja FGFR1 je pokazao umjerenu reaktivnost, dok su FGFR2 i VEGF pokazali blagu reaktivnost. U tkivu karcinoma bubrega visokog stupnja, svi biljezi su pokazali umjerenu reaktivnost. Utvrđena je kolokalizacija VEGF - a s FGFR1 i FGFR2 u karcinomu bubrega niskog i visokog stupnja. Zaključak: Temeljem rezultata utvrđen je izražaj i kolokalizacija VEGF, FGFR1 i FGFR2 biljega u različitim stadijima karcinoma bubrežnih stanica i u normalnom bubrežnom tkivu. Navedeni rezultati doprinose boljem razumijevanju uloge ovih čimbenika u različitim patohistološkim stadijima karcinoma bubrega.
Abstract (english)
Introduction: Renal cell carcinoma (RCC) is the most common type of kidney cancer with a continuous increase in incidence and mortality in the world. Therefore, the efforts of numerous research groups are focused on the detection of significant diagnostic, prognostic and therapeutic markers that would improve the quality of health care for patients with renal cell carcinoma. Objectives: The aims of our study were to determine the expression of VEGF, FGFR1 and FGFR2 markers in different stages of renal cell carcinoma in regard to the expression in normal tissue and to determine the colocalization of VEGF with FGFR1 and FGFR2 in renal cell carcinoma considering the pathohistological tumor grade. Materials and methods: The study was performed on samples of tumor tissue and normal renal tissue, taken from respondents diagnosed with low (5 samples) and high grade (5 samples) renal cell carcinoma according to the WHO. Double imunofluorescence staining of renal cell carcinoma tissue sections was performed and controlled on combination of VEGF with FGFR1 and FGFR2. The percentage of positive cells was calculated on ten representative fields from each section and expressed as mean ± SD and in the relationship between the groups (low and high stage and control group). We used the Kruskal-Wallis and Dunn posthoc test for data analysis. Results: The results of our study showed a positive immunohistochemical expression of VEGF, FGFR1 and FGFR2 in low and high grade renal cell carcinoma tissue, as well as in normal renal tissue. Occasional colocalization of FGFR1 and VEGF in normal renal tissue and colocalization of FGFR1 and FGFR2 with VEGF in low and high grade renal carcinoma, was established. In normal renal tissue, FGFR1, FGFR2 and VEGF showed mild reactivity. In low grade renal cell carcinoma tissue, FGFR1 showed moderate reactivity, while FGFR2 and VEGF showed mild reactivity. In high grade renal cell carcinoma tissue, all markers showed moderate reactivity. The colocalization of VEGF with FGFR1 and FGFR2 in low and high grade renal carcinoma, was determined. Conclusion: Based on the results, the expression and colocalization of VEGF, FGFR1 and FGFR2 markers in different stages of renal cell carcinoma and in normal renal tissue, were determined. These results contribute to a better understanding of the role of these factors in different pathohistological stages of renal cell carcinoma.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:171:925999
Study programmeTitle: Dental Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica dentalne medicine (doktor/doktorica dentalne medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2020-11-17 12:05:53