Objectives: Qualitative analysis of the composition of eye drops, comparison of results with declarations on packaging and determination of possible connection between presence of found ingredients and allergic reactions. Material and methods: In this study, 7 samples of eye drops were analyzed, of which 4 in prescripiton and 3 in over-the-counter dispensing mode. In analysis preparation glass Petri dishes with a 100 μL sample were used. Samples were extracted with a mixture of organic solvents: chloroform, ethyl acetate and N-hexane (v / v / v = 1: 1: 1). The samples were then placed in a fume hood for drying in a stream of air. Samples in Petri dishes were washed several times with chloroform to dissolve the entire content. After dissolution samples were filtered and transferred to glass cuvettes for GC-MS analysis. Results: Out of 7 analyzed samples, in only 2 samples (samples 1 and 3) were detected traces of some substances - organic solvents. Benzalkonium chloride was mentioned as a preservative on the declaration in four analyzed samples, however, BAK was not detected by the method used. Other preservatives that according to the literature cause allergic reactions: parabens, thiomersal and chlorhexidine are not listed in the composition of the analyzed eye drops, nor were they detected by the method used. Neomycin, which is listed as a common sensitizer, is the active substance in sample 6, but was not found by the GC-MS method used. Other previously mentioned causes of allergic reactions in eye drops are not listed on the declarations of the analyzed samples, nor were they detected by instrumental analysis. Conclusions: Compounds detected by this study may be due to contamination of the samples during analysis. Benzalkonium chloride is the most common allergen present in eye drops, the GC-MS method used did not detect it, however, this does not rule out the possibility that it was present but suggests that the method used was probably not sensitive enough or its concentration was low. The most appropriate method for the qualitative and quantitative determination of benzalkonium chloride in eye drops according to eralier mentioned studies would be HPLC. Other allergens present in eye drops have not been sufficiently investigated, therefore further researches are needed with appropriate methods.